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strings dna fragment synthesis service  (Thermo Fisher)


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    Thermo Fisher strings dna fragment synthesis service
    Strings Dna Fragment Synthesis Service, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 468036 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strings dna fragment synthesis service/product/Thermo Fisher
    Average 99 stars, based on 468036 article reviews
    strings dna fragment synthesis service - by Bioz Stars, 2026-04
    99/100 stars

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    strings dna fragment gene synthesis service  (Thermo Fisher)
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    Thermo Fisher strings dna fragment gene synthesis service
    Schema for construction of S100A8/A9 coexpression vector and their protein expression in E. coli. (A) Expression vector for S100A8 <t>or</t> <t>S100A9</t> were reconstructed into coexpression vectors by PCR amplification of each open reading frame and then subcloned by recombinase reaction. (B) Recombinant protein expression by each plasmid <t>DNA</t> for pET21-S100A8 (Lane 1), pET21-S100A9 (Lane2), pET21-S100A8-S100A9 (Lane 3), and pET21-S100A9-S100A8 (Lane 4) were confirmed by SDS-PAGE. Gels were stained with Coomassie Brilliant Blue (CBB).
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    Schema for construction of S100A8/A9 coexpression vector and their protein expression in E. coli. (A) Expression vector for S100A8 or S100A9 were reconstructed into coexpression vectors by PCR amplification of each open reading frame and then subcloned by recombinase reaction. (B) Recombinant protein expression by each plasmid DNA for pET21-S100A8 (Lane 1), pET21-S100A9 (Lane2), pET21-S100A8-S100A9 (Lane 3), and pET21-S100A9-S100A8 (Lane 4) were confirmed by SDS-PAGE. Gels were stained with Coomassie Brilliant Blue (CBB).

    Journal: Biochemistry and Biophysics Reports

    Article Title: An efficient method for the preparation of preferentially heterodimerized recombinant S100A8/A9 coexpressed in Escherichia coli

    doi: 10.1016/j.bbrep.2016.03.009

    Figure Lengend Snippet: Schema for construction of S100A8/A9 coexpression vector and their protein expression in E. coli. (A) Expression vector for S100A8 or S100A9 were reconstructed into coexpression vectors by PCR amplification of each open reading frame and then subcloned by recombinase reaction. (B) Recombinant protein expression by each plasmid DNA for pET21-S100A8 (Lane 1), pET21-S100A9 (Lane2), pET21-S100A8-S100A9 (Lane 3), and pET21-S100A9-S100A8 (Lane 4) were confirmed by SDS-PAGE. Gels were stained with Coomassie Brilliant Blue (CBB).

    Article Snippet: The gene fragments encoding human S100A8 (Uniprot: P05109 ) and S100A9 (Uniprot: P06702 ) were prepared by GeneArt Strings DNA fragment gene synthesis service (Life Technologies) with the sequence optimized for E. coli protein expression.

    Techniques: Plasmid Preparation, Expressing, Amplification, Recombinant, SDS Page, Staining